Proteomics Protocols and
Selected Reading

Protocols

Manual Digestion Protocol

Urea Solution Digest Protocol

Methanol-Chloroform Precipitation Protocol

Selected Reading

• Mann, M., Kelleher, N.L., Precision Proteomics: The case for high resolution and high mass accuracy, Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18132-8

• Graumann, J., et. al., SILAC-labeling and proteome quantitation of mouse embryonic stem cells to a depth of 5111 proteins, Mol Cell Proteomics. 2008 Apr;7(4):672-83. Epub 2007 Nov 28.

• Haas, W., et. al., Optimization and use of peptide mass measurement accuracy in shotgun proteomics, Mol Cell Proteomics. 2006 Jul;5(7):1326-37. Epub 2006 Apr 23.

• Walker, J.M., The Proteomics Protocols Handbook, 2005

• Simpson, R.J., Purifying Proteins for Proteomics: A Laboratory Manual, 2004