Genomics Services
Sequencing
Sanger
Custom and High throughput
Bacterial and Purified
Next Gen - Illumnia
GAII and Hi Seq 2000
Next Gen Roche 454
Ion torrent
DNA Genotyping
Fragment Analysis with ABI 3130 Capillary Instrument
Mouse "Tail-snip" Genotyping
QPCR
The RTSF has the ABI Prism 7900HT Sequence Detection System for real-time quantitation of gene expression and for detection of single nucleotide polymophisms (SNPs). This system gives researchers the power to perform precise quantitative nucleic acid assays. PCR quantitation can be carried out using either the TAQman primer-probe or SYBR green chemistries. The 7900HT system offers 384-well capacity and robotic loading for high throughput capability.
Array Services
Affymetrix
Agilent
Custom Spotted Arrays
Microarray
Illumina Bead Express
Other Services Offered
Covaris S2 Adaptive Focused Acoustic Disruptor
The Covaris S2 Adaptive Focused Acoustic Disruptor enables improved preparation of small biological samples up to 100ul in volume. Its capabilities include DNA and chromatin shearing, tissue disruption and homogenization, and cell lysis among others.
The benefits of this method of preparation compared to standard sonication and homogenization techniques are:
Isothermal processing which minimizes heat damage.
Non-contact, closed vessel processing eliminates cross contamination problems.
Precisely controlled energy delivery allows standardized, highly reproducible processing of samples.
